A computer with Unix, Linux or Mac OS X operating systems.Quote from that publication: Necessary Resources Hardware The memory needed to run the tool line-command is the same as when running it within Galaxy. 16 GB is not sufficient for human mapping. Yes, the Gencode GTF looks to be not the problem. Gencode or iGenomes are both good choices for human/mouse, with iGenomes supporting even more genomes. Use one of the other sources linked above instead for best results. This effectively means that all counts or other summaries that are putatively “by gene” are actually “by transcript” (a scientific content problem).
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